PERSIAN Biobank
Developing a biobank— a type of repository storing biological samples—has become an important part of clinical research in the past few decades. If stored under the appropriate conditions, a variety of biological specimens can be kept for many years, and be used for research in medical, epidemiological, and genetic studies, ideally to identify new biomarker, onco-markers or genes causing disease in a large section of the population.
A variety of samples are stored in the PERSIAN Cohort biobank. Samples are taken at enrollment. If one of the study’s main outcomes of interest is noted in the follow up phase (cancer or other disease development), additional blood samples are taken and stored. A fraction of aliquoted samples are stored in local biobanks at the cohort centers, and a fraction is stored centrally in the PERSIAN Cohort Central Biobank. The PRESIAN Cohort Laboratory Information Management System is designed and used for easy tracking and access to samples.
Types of samples collected and stored in the PERSIAN Cohort Biobanks
Blood
Twenty milliliters (20 cc) of blood is taken from each participant (fasted). A portion of the serum is used to run the following biochemistry tests:
- Complete blood count
- Fasting blood sugar
- Total cholesterol
- HDL cholesterol
- Triglycerides
- Alanine transaminase
- Aspartate transaminase
- Alkaline phosphatase
- Gamma-glutamyl transpeptidase
- Blood urea nitrogen
- Creatinine
Results obtained from the tests above are stored in the PERSIAN Cohort databases and also given back to participants as an incentive.
The majority of the blood taken is aliquoted and stored in -70C freezers. Blood is stored as whole blood, red blood cells, plasma, serum and buffy coat.
Urine
Urine is collected from each individual and stored in -30C freezers. A urine analysis is also performed, the results of which are recorded in the cohort database and also returned to participants.
Hair and Nail
All finger and toenails as well as 200-300 strands of hair are collected from each participant. Samples are placed inside foil wraps, which are then placed in individual zipped bags along with a desiccant de-humidifier, and kept in cool, dry storage.
Other Samples
In addition to the above samples, the PERSIAN Birth Cohort collects additional samples such as cord blood, maternal milk, stool, and baby’s first tooth from a subset of participants.
Overview of samples available in the PERSIAN Cohort Biobank
PERSIAN Cohort (Main-adult)
Cohort Study | Date Started | No. Recruited | Samples |
Azar | 2014 | 15,006 |
Blood: 2,484,510 aliquots For each individual: o Whole Blood: two 1.5 ml and one 1 ml cryotubes o Plasma: two 1.5 ml and five 1ml cryotubes o Buffy coat: three 0.5-1 ml cryotubes o Serum: two 1 ml cryotubes
Urine: 331,268 aliquots Hair: 165,634 samples Nail: 165,634 samples |
Fasa | 2014 | 10,153 | |
Guilan | 2014 | 10,521 | |
Ravansar | 2014 | 10,048 | |
Kharameh | 2014 | 10,663 | |
Yazd | 2015 | 10,005 | |
Tabari | 2015 | 10,255 | |
Rafsanjan | 2015 | 10,614 | |
Zahedan | 2015 | 10,085 | |
Hoveyzeh | 2016 | 10,009 | |
Shahrekord | 2016 | 10,116 | |
Bandare Kong | 2016 | 4,076 | |
Urmia Lake | 2017 | 5,168 | |
Ardabil | 2017 | 20,985 | |
Kavar | 2017 | 4,997 | |
Sabzevar | 2017 | 5,275 | |
Dena | 2018 | 3,662 | |
Dehgolan | 2019 | 3,996 | |
Total |
| 165,634 |
|
PERSIAN Birth Cohort
Cohort Study | Date Started | No. Recruited | Samples |
Yazd | 2015 | 3,110 | Blood: 165,300 aliquots Urine: 22,040 aliquots Hair: 11,020 samples Nail: 11,020 samples |
Isfahan | 2016 | 3,755 | |
Semnan | 2017 | 1,027 | |
Sari | 2017 | 2,000 | |
Rafsanjan | 2019 | 1,128 | |
Total |
| 11,020 |
|
PERSIAN Youth Cohort
Cohort Study | Date Started | No. Recruited | Samples |
Fasa | 2014 | 3,014 | Blood: 173,880 aliquots Urine: 23,184 aliquots Hair: 11,592 samples Nail: 11,592 samples |
Ravansar | 2014 | 2,991 | |
Rafsanjan | 2015 | 3,006 | |
Sari | 2016 | 2,581 | |
Total |
| 11,592 |
|
PERSIAN Elderly Cohort
Cohort Study | Date Started | No. Recruited | Samples |
Neyshabour | 2016 | 7,465 | Blood: 248,475 aliquots Urine: 33,130 aliquots Hair: 16,565 samples Nail: 16,565 samples |
Guilan | 2018 | 3,700 | |
Tabriz | 2019 | 1,400 | |
Ardakan | 2019 | 4,000 | |
Total |
| 16,565 |
PERSIAN Employee Cohort (Ongoing – Updated Jan 2022)
Cohort Study | Date Started | No. Recruited | Samples |
Mashhad (including Sangan) | 2017 | 8,612 |
|
Rafsanjan | 2018 | 2,111 | Blood: 338,520 aliquots Urine: 45,136 aliquots Hair: 22,568 samples Nail: 22,568 samples |
Shiraz | 2018 | 5,877 | |
Tabriz | 2018 | 2,906 | |
Shahroud | 2019 | 1,178 | |
Qom | 2019 | 319 | |
Qazvin | 2019 | 1,086 | |
Zahedan | 2020 | 196 | |
Birjand | 2021 | 283 | |
Total |
| 22,568 |
|