The PERSIAN Cohort Biobank
Developing a biobank— a type of repository storing biological samples—has become an important part of clinical research in the past few decades. If stored under the appropriate conditions, a variety of biological specimens such as blood, urine, stool, hair, nail, etc. can be kept for many years, and be used for different kinds of medical, epidemiological, and genetic researches. The data gathered in a biobank is ideal for studies needing to test something, such as a new biomarker or gene, in a large section of the population.
After successfully creating a biobank for the Golestan Cohort Study, a cohort of 50,000 individuals in north eastern Iran, a biobank has also been designed for the PERSIAN Cohort, which consists of blood, urine, hair, and nail samples of over 170,000 Iranians from 17 provinces in Iran, and from 8 different ethnicities.
PERSIAN Cohort Blood and Urine Specimens
Twenty milliliters (20 cc) of blood is taken from each individual (fasted). A small portion is used to run biochemistry tests such as complete blood count, fasting blood sugar, total cholesterol, HDL cholesterol, triglycerides, alanine transaminase, aspartate transaminase, alkaline phosphatase, gamma-glutamyl transpeptidase, blood urea nitrogen, and creatinine levels, the results of which are stored in the database and also given back to participants as an incentive. The rest of the blood is aliquoted and stored in -70C freezers. Blood is stored as whole blood, red blood cells, plasma, serum and buffy coat.
Urine is also collected from each individual and also stored in -70C freezers, results of a urine analysis are also recorded in the database and returned to participants.
All biological samples are labeled using barcodes unique to each individual, and placed inside Cryo Boxes, prior to placement in freezers. The lab personnel use a software to identify the exact location of each sample so that retrieving them can be done in a short amount of time.
PERSIAN Cohort Hair, and Nail Specimens
All finger and toenails as well as 200-300 strands of hair (10 centimeters long) are collected from each participant. Samples are placed inside foil wraps, which are then placed in individual zipped bags, and kept in a cool and dry place.
|Prospective Epidemiological Research Studies in IrAN (PERSIAN Cohort)|